|
Column
|
Waters HSS T3 (1.8 μm)
|
|
Injection volume
|
10 μL
|
|
Oven temperature
|
40 °C
|
|
Mobile phase A
|
Water + 10 mM ammoniumacetate
|
|
Mobile phase B
|
Acetonitrile + 0.1% formic acid
|
|
Flow
|
0.4 mL/min
|
|
Gradient
|
0–0.25 min 2% solvent B
|
|
0–7 min linear gradient to 98% solvent B
|
|
7–8 min 98% solvent B
|
|
8–9 min linear gradient to 2% solvent
|
|
9–10 min 2% solvent B
|
|
Detector
|
Triple quadrupole mass spectrometer
|
|
Spectrometer
|
None
|
|
Interface
|
Electrospray ionisation
|
|
Potential
|
5000 V
|
|
Temperature
|
500 °C
|
|
Scan type
|
MRM (Multiple Reaction Monitoring Mode)
|
|
Collision gas
|
Argon
|
