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Table 1 Performance parameters and mass detector settings for the assessed analytes in order of increasing molar mass

From: Chemical assessment of mycotoxin contaminants and veterinary residues in Costa Rican animal feed

Analyte Molar mass, g/mol Retention time, min Target ion, m/z Fragmentor Dwell time, ms Calibration curve equationa or response factor bLimit of detection, μg kg− 1 (cμg L− 1) Limit of quantification, μg kg− 1
Mycotoxin contaminants
 Patulin 154.21 24.63 154.3 [M+] 120 71 y = (4.81 × 103 ± 2.69 × 102)x 0.62 (10.1) 1.84
 Citrinin 251.10 23.48 251.0 [M+] 120 56 y = (3.67 × 103 ± 8.11 × 102)x 0.34 (1.9) 1.03
 Deoxynivalenol 296.30 8.69 297.1 [M + H]+ and 319.0 [M + Na]+ 120 144 y = (1.61 × 103 ± 4.11 × 102)x 6.31 (105.2) 19.12
 Aflatoxin B1 312.28 21.11 313.0 [M + H]+ 140 34 y = (2.62 × 102 ± 3.14 × 101)x 0.40 (6.7) 1.21
 Nivalenol 312.32 5.14 355.1 [M + 2Na]+ 140 143 y = (1.73 × 102 ± 2.10 × 101)x 6.42 (107.0) 19.45
 Aflatoxin B2 314.30 20.48 315.0 [M + H]+ 140 34 y = (1.01 × 102 ± 3.15 × 101)x 0.14 (2.3) 0.42
 Zearalenone 318.40 24.67 319.0 [M + H]+ 120 71 y = (6.75 × 102 ± 3.41 × 101)x 0.26 (4.3) 0.79
 β-zearalenol 320.38 22.16 321.0 [M + H]+ 120 56 y = (1.43 × 102 ± 2.55 × 101)x 0.44 (7.3) 1.33
 α-zearalenol 320.38 23.07 321.1 [M + H]+ 120 56 y = (4.80 × 103 ± 2.01 × 102)x 0.63 (5.67) 1.91
 Sterigmatocystin 324.30 25.43 327.1 [M + 3H]+ 140 95 y = (7.64 × 102 ± 5.54 × 101)x 0.60 (10.0) 1.82
 Aflatoxin G1 328.30 20.48 328.9 [M + H]+ 140 34 y = (5.95 × 102 ± 2.33 × 101)x 0.27 (4.5) 0.82
 Aflatoxin M1 328.27 19.13 329.0 [M + H]+ 140 34 y = (4.02 × 103 ± 3.61 × 102)x 0.005 (0.8) 0.02
 Aflatoxin G2 330.30 19.87 331.0 [M + H]+ 140 34 y = (1.10 × 102 ± 1.12 × 101)x 0.20 (3.3) 0.61
 3-acetoxydeoxynivalenol 338.35 18.01 339.2 [M + H]+ 140 71 y = (1.78 × 102 ± 6.65 × 101)x 0.38 (6.3) 1.15
 15-acetoxydeoxynivalenol 338.35 18.26 339.1 [M + H]+ 120 71 y = (8.77 × 102 ± 2.15 × 101)x 0.30 (5.0) 0.91
 Fusarenon-X 354.35 15.74 355.0 [M + H]+ 140 71 y = (1.40 × 102 ± 1.17 × 101)x 0.29 (4.8) 0.88
 Diacetoxyscirpenol 366.41 16.84 365.0 [C19H25O7]+˙ - H˙ 140 71 y = (8.35 × 101 ± 4.31 × 100)x 0.32 (5.3) 0.97
 Ochratoxin B 369.37 22.71 370.0 [M + H]+ 120 56 y = (1.23 × 102 ± 1.15 × 101)x 0.21 (3.5) 0.64
 Neosolaniol 382.41 20.79 384.2 [M + 2H]+ 140 34 y = (8.74 × 102 ± 9.14 × 101)x 0.61 (10.2) 1.85
 Ochratoxin A 405.40 24.35 404.0 [M + H]+ 120 71 y = (1.19 × 102 ± 5.09 × 101)x 0.16 (2.7) 0.48
 HT-2 toxin 424.48 21.38 425.1 [M + H]+ 140 56 y = (1.06 × 103 ± 2.19 × 102)x 0.32 (5.3) 0.97
 T-2 toxin 466.47 22.66 484.3 [M + NH4]+ 140 71 y = (8.78 × 102 ± 4.54 × 101)x 0.96 (16.0) 2.91
 Enniatin B 639.83 29.94 640.3 [M + H]+ 120 95 y = (3.50 × 102 ± 1.07 × 101)x 1.16 (19.2) 3.50
 Enniatin B1 653.86 30.98 654.3 [M + H]+ 120 95 y = (2.00 × 103 ± 2.01 × 102)x 0.11 (1.8) 0.34
 Fumonisin B1 721.84 18.88 723.2 [M + 2H]+ 100 34 y = (1.19 × 103 ± 1.27 × 102)x 0.08 (1.3) 0.24
 Fumonisin B2 705.84 20.09 706.2 [M + H]+ 100 34 y = (3.20 × 102 ± 3.23 × 101)x 0.43 (7.17) 1.30
Veterinary residues
 Sulfamethazine 278.33 9.18 279.0 [M + H]+ 120 146 2.61 × 104 ± 6.91 × 103 45.45 (50) 151.52
 Spectinomycin 332.35 1.18 333.1 [M + H]+ 140 97 7.53 × 102 ± 1.91 × 101 68.18 (75) 227.27
 Florfenicol 358.20 13.15 339.9 [C12H14Cl2NO4S]+˙ - F˙ 120 97 1.33 × 102 ± 2.32 × 101 45.45 (50) 151.52
 Cloramphenicol 323.13 14.51 344.9 [M + Na]+ 120 97 3.02 × 102 ± 9.11 × 101 46.36 (51) 154.55
 Penicillin G 334.39 17.28 354.0 [M + Na]+ and 367.0 [M + 2H2O]+ 60 83 1.75 × 103 ± 2.67 × 102 97.27 (107) 324.24
 Lincomycin 406.54 7.23 407.1 [M + H]+ 120 146 4.32 × 103 ± 1.41 × 102 81.82 (90) 272.73
 4-epianhydrotetracycline 426.42 14.77 427.0 [M + H]+ 120 97 1.27 × 103 ± 5.44 × 102 100.01 (105) 303.03
 Methacycline 442.42 13.68 443.0 [M + H]+ 120 97 2.33 × 102 ± 7.83 × 101 57.27 (63) 190.91
 Tetracycline 444.44 10.12 445.0 [M + H]+ 120 97 2.00 × 103 ± 4.23 × 102 41.82 (46) 139.39
 Doxycycline 444.44 14.08 446.0 [M + 2H]+ 120 97 1.17 × 102 ± 5.67 × 101 83.64 (92) 278.79
 4-epitetracycline 444.44 10.23 447.1 [M + 3H]+ 140 97 3.19 × 102 ± 3.23 × 101 90.91 (100) 275.48
 Minocycline 457.48 7.14 458.1 [M + H]+ 120 146 1.25 × 103 ± 1.01 × 102 71.21 (83) 254.38
 Demeclocycline 464.85 11.43 465.0 [M + H]+ 120 97 1.12 × 103 ± 7.34 × 102 41.82 (46) 139.39
 Oxytetracycline 460.44 9.46 461.0 [M + H]+ 140 97 2.27 × 102 ± 6.51 × 101 82.73 (91) 275.76
 4-epioxytetracycline 460.44 9.42 462.3 [M + 2H]+ 120 146 2.94 × 102 ± 2.21 × 101 101.02 (113) 368.63
 Chlortetracycline 478.88 13.10 479.0 [M + H]+ 120 97 1.25 × 103 ± 5.55 × 102 92.12 (102) 301.06
 4-epichlortetracycline 478.88 11.57 479.1 [M + H]+ 120 97 6.51 × 102 ± 3.48 × 101 132.73 (146) 442.42
 Virginimycin M1 525.60 21.94 526.1 [M + H]+ 120 83 4.46 × 103 ± 2.46 × 102 59.09 (65) 196.97
 Monensin A 670.88 24.56 693.2 [M + Na]+ 180 83 1.13 × 104 ± 1.01 × 103 40.91 (45) 136.36
 Erythromycin 733.94 16.83 734.3 [M + H]+ 140 83 1.16 × 104 ± 1.76 × 103 80.93 (89) 264.56
 Narasin 765.04 25.20 787.3 [M + Na]+ 260 83 1.26 × 103 ± 2.26 × 102 24.55 (27) 81.82
 Virginimycin S1 823.90 23.35 824.1 [M + H]+ 120 83 3.47 × 103 ± 3.44 × 102 59.09 (65) 196.97
 Tylosin 916.11 18.05 916.2 [M+] 140 83 5.37 × 103 ± 2.88 × 102 45.45 (48) 151.52
  1. aAll trend line intercepts adjusted to zero as their values are deemed negligible with respect to the slopes and areas. The equations shown are the result of an average of three curves measured independently and under reproducibility conditions
  2. bLimit of detection and quantification calculated as S/N ratio times 3.3 and 10, respectively. Values corroborated by extinction assays
  3. cSmallest concentration detected for each analyte within the calibration curve (experimental data)