Ochratoxin A in black pepper, white pepper and clove sold in Yaoundé (Cameroon) markets: contamination levels and consumers’ practices increasing health risk

Abstract

Background

Spices are widely consumed in Cameroon, but there is no data available concerning their mycotoxin contamination. A cross-sectional study was conducted from February to June 2017 to assess the occurrence of Ochratoxin A (OTA) in black pepper, white pepper and clove sold in three main markets of Yaoundé (the capital of Cameroon), and the health risk that can be associated taking into account consumers’ practices during and after purchase.

Results

Data obtained revealed that 10% of black pepper samples and 40% of the white pepper samples contained OTA at levels ranging between 1.15–1.91 μg/Kg and 1.81–4.89 μg/Kg, respectively. This is below the established European Commission regulated limit of 15 μg/Kg in spices. This toxin was not detected in clove samples. Considering the consumption data collected from our survey, the maximum OTA daily intake was estimated at 0.182 and 0.699 ng/kg bw/day for black pepper and white pepper, respectively. This is lower than the EFSA tolerable value of 17 ng/kg bw/day. However, it also comes out that 88% of respondents did not have an idea of mycotoxins. Furthermore, 5–20% of them do not pay attention to some general quality indicators such as odour, colour and presence of moulds reported by the others when purchasing or storing the three spices, the latter being the most pertinent indicator of the possible presence of mycotoxins. These spices are generally stored at room temperature and for prolonged periods, conditions which are suitable for fungal growth and mycotoxin production.

Conclusion

Although the present study shows limited or no health risk associated to OTA contamination in black pepper, white pepper and clove, it is necessary to enhance consumers’ awareness on mycotoxins and their associated impact on health, and to sensitise them on preventive measures to be taken.

Background

Spices are agricultural products cultured or picked in the wild. They can be seeds (coriander), fruits (pepper and mustard), flowers (saffron and clove), or shoots (skin, roots, and leaves) of several plants that have indigenous or exotic origin and with a strong aroma and taste. They are widely used in daily diet preparations in order to enhance colour, taste, smell, or flavour (Ayres et al., 1980; Anonim 2000; Bulduk 2004).They have also been used as raw materials in pharmaceutical preparations and supplements for dietetic products, especially for “self-medications”. Nowadays, due to their preservative and antioxidant properties, spices are widely used for many industrial applications, especially in the food industry (El-Gali 2014).

Even though spices are generally used in small amounts, they represent an important vector of microbial contamination due to the conditions under which they were grown, harvested and processed (Romagnoli et al. 2007; Gnonlonfin et al. 2013).Fungi are the most represented microbial flora isolated from these products. They are able to grow on, and this may subsequently lead to mycotoxin contamination (Kocic-Tanackov et al., 2007). Mycotoxins are a group of secondary metabolites produced by certain fungi that can grow on foodstuffs including spices under certain environmental conditions (Dancea et al. 2004; Arshad and Muhammad, 2012), especially in tropical climates (high ranges of temperature, humidity and rainfall) which are suitable for this toxic metabolites production (Bokhari 2007). Their presence at a certain amount in food can be very harmful for consumers. More than 400 mycotoxins have been identified up to date among which aflatoxins (AFs), fumonisins and ochratoxins are the most studied (Righetti et al. 2016). Ochratoxin A (OTA) or L-phenylalanylcarbonyl-5-chloro-8-hydroxy-3,4-dihydro-3-R-methylisocoumarin (Fig. 1) is the most detected mycotoxin in human blood all over the world (Pena et al. 2006). It is mainly produced by fungi belonging to the Aspergillus and Penicillium genera (Amézqueta et al. 2012), principally A. ochraceus and P. verrucosum (Sibanda et al. 2002). Studies to establish its toxicity showed that this toxin is carcinogenic, teratogenic, genotoxic, immunotoxic, nephrotoxic and neurotoxic (Ringot et al. 2006). The International Agency for Research on Cancer (IARC) of the World Health Organization (WHO) has classified OTA as toxic for humans (group 2B carcinogen) (IARC 1993). Because of its health impact on humans and animals coupled with its wide distribution in various agricultural commodities, this toxin is considered among the most significant mycotoxins, which are subject to regular inspection.

Fig. 1

Chemical structure of Ochratoxin A

Taking into consideration the potential health risk associated with OTA, the presence of this mycotoxin in spices has been investigated worldwide. Aziz et al. (1998) reported the presence of OTA in spices collected from various retailers in the city of Cairo (Egypt). Toma and Abdulla (2013) found that OTA was present in spices sold in Iraqi markets in the Erbil city. Salari et al. (2012) also reported the presence of OTA in spices collected from a farm and sun-dried in Iran. Although interest on investigating OTA contamination in spices in other parts of the world has risen, no such studies have been carried out in Cameroon despite the wide use of a plethora of spices in the country. This study aimed at evaluating the occurrence of OTA in some selected spices sold in Yaoundé markets (white pepper, black pepper and clove), and the health risk that can be associated taking into account consumers’ practices during and after purchase.

Methods

Study site

This study was carried out between February and June 2017 in Yaoundé (Centre Region of Cameroon), a multicultural city where black pepper (Piper nigrum L.), white pepper (Piper nigrum L.) and clove (Syzygium aromaticum L.) coming from different regions of the country and from other countries are sold.

Survey

A total of one-hundred Cameroonians living in Yaoundé were selected to take part in this field survey based on their ease of access. These participants selected were people who usually buy food spices including black pepper, white pepper and clove. A structured questionnaire was administered and it primarily aimed at retrieving information personally from participants that could assist in (i) assessing the frequency and use of these spices; (ii) identifying their quality indicators and the preservation methods used; (iii) evaluating the awareness of the population on risks associated to mycotoxins in spices, and handling measures adopted after purchasing the targeted spices.

Sampling

Samples (approximately 100 g each) of black pepper, white pepper and clove were randomly purchased from different sellers at the main markets of Yaoundé (Mokolo, Mfoundi and Mendong markets). The samples collected were taken to the laboratory in condition under which they were sold (sealed plastics in general), and dried at 50 °C for 3 days. Prior to the OTA analysis, the dried samples were ground in a blender (IKA A11 basic; IKA-WerkeGmbH & Co. KG, Staufen, Germany) to pass through a 500 μm sieve.

Ochratoxin A determination

Ochratoxin A content in each spice was determined using a quantitative ELISA (Enzyme Linked Immounosorbent Assay) kit for Ochratoxin A in Coffee, Cocoa, and Spices (CAT. N° 961OCH01COF Qual, HELICA Biosystems, Inc., Santa Ana, CA). The extraction was conducted following the manufacturer’s protocol. For each sample, 10 g of the powder obtained was added to 50 mL of methanol (LC grade, Merck, Darmstadt, Germany) diluted at 70%. The mixture was then homogenized for 5 min using a vortex (Vortex Genius 3, IKA, Germany) and centrifuged (Eppendorf Centrifuge 5702) at 2000 g for 10 min. The supernatant obtained was used for the Competitive Direct Enzyme Linked Immunosorbent Assay (CD-ELISA). The OTA content was inversely proportional to the colour intensity established using an automated microplate reader (EL × 800, BIOTEK, Instruments Inc., Winooski, VT, United States) at 450 nm. OTA content in each sample was calculated based on a calibration curve that was plotted in each experiment using the OTA standard at different concentrations (0, 2, 5, 10, 20 and 40 ppb). The correlation coefficients were above 0.99. The lower and upper limits of quantification of the kit were 1 and 20 ppb (μg/kg), respectively, according to the supplier’s information. Samples with toxin values below the limit of quantification (LOQ) were recorded as containing no detectable toxin.

Assessment of the exposure to OTA by the studied spices

A deterministic risk analysis was performed as described by Coronel et al. (2011). OTA daily intake (ng OTA/kg bw/day) was estimated by multiplying the spice OTA concentration (ng/g) by spice individual consumption data (g/kg bw/day) obtained from the survey. Calculations were done assuming an average populations body weight of 60 kg.

Statistical analysis

The statistical software Sphinx Plus² Lexica was used to generate the questionnaire and analyze the obtained data. The OTA content in the different spices was expressed as mean ± standard deviation and Statistica10 software of Statsoft used to perform a one-way ANOVA to establish significant differences between the concentration levels obtained (p ≤ 0.05). This software was also helpful in generating min, max, mean, P75, P90 and P95 OTA daily intake per spice.

Results

Socio-demographic and perception study findings

Table 1 presents the descriptive statistics of data collected from the survey. The 100 participants (15 men and 85 women) had in common the habit of purchasing the three selected spices. They were from different Cameroonian tribes. Their age was principally in the interval 21–35 years old (66%) and above (25%). Most of them were single (52%) and 43% married. Their academic level ranged from primary level (17%), high school level (32%) to university level (47%). Only 4% did not have an academic background. Some of them were housekeepers (21%), students (25%) and the majority was workers (44%).

Table 1 Descriptive statistics of data collected from the survey (n = 100)

It appears that the three spices are mainly used for cooking purpose as condiments (in large quantities to supplement diets) during food preparations or in low amounts to impart or enhance flavour. For instance, they have been described to be used in the preparation of some traditional meals and beverages, tomatoes and groundnuts sauces, fried eggs etc. The use of spices in folklore medicine has also been described. For example, clove has been described as useful in case of tooth pain, stomach pain, stress, bad breath, cold and to facilitate childbirth. Only a few medicinal properties were reported for black and white peppers. Black pepper is found useful to calm stomach pain by 5% of the people interviewed, and 1 to 2% declared that consuming soup, which contained black and white pepper facilitates childbirth.

Concerning the consumption frequency, white pepper is clearly the spice consumed at the highest frequency (at least thrice a week) when compared to the others under study that rather tended to be rarely consumed (at most once a week). An estimation of the average quantities of spices (per food preparation) that can be associated to this consumption frequency shows that quantities below 5 g are generally used. At quantities above 10 g, white pepper is the most represented.

In terms of quality criteria and preservation methods used, the respondents appreciate the quality of spices during purchase and at storage in homes. Colour and odour were the main attributes of spices taken into account by 48–58% and 73–77% of respondents (depending on the spice) respectively, with taste and texture as secondary. The presence of visible moulds and humidity of the spices are also considered during storage at home. It also appears that 5 to 20% of respondents do not pay attention to these attributes when purchasing or preserving these spices.

The purchased spices are generally consumed throughout if available. The duration of storage at home as reported generally ranges from 0 to 8 weeks and in some cases, can even extend beyond 12 weeks. It is mainly done at room temperature in plastic or glass bottles.

Public awareness about the potential presence of moulds and mycotoxins in spices was also assessed. From this survey, it could be noticed that 94% of respondents knew about moulds but 88% of them did not know that mould contamination may imply the presence of mycotoxins. For those who were aware, they reported that they take particular measures such as drying spices to limit mould contamination and to wash them before use.

Ochratoxin A contamination and exposure level

The Table 2 presents the results obtained from the determination of OTA in the spices samples. Positive samples (OTA content above the 1 μg/kg LOQ) were observed in black and white peppers. With black pepper, only 10% (2 over 20) of the samples were positive (mean: 1.53 ± 0.54 μg/kg; max: 1.91 μg/kg), whereas up to 40% (8 over 20) of white pepper samples contained OTA at levels (mean: 3.30 ± 1.00 μg/kg; max: 4.89 μg/kg) that were significantly higher than those of black pepper (p = 0.047). No presence of OTA was detected in clove samples.

Table 2 Ochratoxin A levels in the spices samples

Based on these OTA contamination data and on spices consumption data, the OTA daily intake (min, max, mean and percentiles 75, 90, 95) was calculated for black pepper and white pepper (Table 3). The different values obtained with both spices were very low (almost null), and this, even in our worst-case scenario where an OTA contamination at the highest content observed in each spice were assumed. Although being weak, this toxin daily intake was higher with white pepper than with black pepper. The maximum was estimated at 0.182 ng OTA/kg bw/day for black pepper, and 0.699 ng OTA/kg bw/day for white pepper.

Table 3 Ochratoxin A daily intake associated to the spices consumption

Discussion

Black pepper, white pepper and clove are used worldwide for food and beverages preparation (Babajide et al. 2002; Mann 2011; Sarkar et al. 2015). Many health benefits, including the therapeutic values reported by people we investigated, have been described for clove (Heinrich and Barnes 2004; Jirovetz et al. 2006; Chaieb et al. 2007), and for black pepper and white pepper (Sofowora 1982; Achinewhu et al. 1995; Noumi et al. 1998; Udoh et al. 1999; Mbongue et al. 2005; Ahmad et al. 2012). However, these spices can also be harmful to consumers in case of contamination by mycotoxins such as OTA.

From our analysis, OTA was detected in black and white pepper samples but at levels below the maximum regulatory limit for this toxin in spices set by the European Commission (EC N°594/2012) which is 15 μg/kg. Indeed, growth of ochratoxinogenic moulds in these two spices has previously been reported in literature (Karan et al. 2005; Toma and Abdulla, 2013).The OTA levels obtained from the white pepper analysed in this study are in general below those reported by Jalili et al. (2010) in white pepper from various market outlets in Malaysia (0.15–13.58 μg/kg). As for levels of OTA obtained in black pepper samples in this study, they are also lower than those reported by Jalili (2016) in similar samples imported into Iran (3.31 ± 2.69 μg/kg) and by Toma and Abdulla (2013) in samples collected from a common market in Erbil city, Iraq (5.8 μg/kg).This suggests a difference in the agricultural or manufacturing (handling, packaging and storage) practices, or the variability of the fungal profile. Besides, as also reported by Jalili (2016), the difference between the environmental conditions (which is an important factor affecting mycotoxin contamination) can also explain the difference between these results. The absence of OTA noticed in clove samples could be related to the antifungal activity of this spice. Mostafa et al. (1990) and Hua et al. (2014) observed the inhibitory effect of clove on fungal growth and OTA production. The absence of OTA in clove has also been reported by Karan et al. (2005) in clove sold at Belgrade, Serbia. Despite having isolated mycotoxin-producing moulds from clove sold in Saudi Arabia, Bokhari (2007) also did not detect the presence of any mycotoxin in this spice.

As observed in this study, white pepper, the spice with the highest OTA content is the most consumed and at the highest frequency. Hopefully, an estimation of the OTA daily intake even with the maximum OTA content detected in positive samples gave values that were far below the Provisional Tolerable Daily Intake (PTDI) value of 17 ng OTA/kg bw/day (EFSA, 2006). Furthermore, the almost null values obtained by evaluating the high percentiles of intake (P75, P90, P95) suggest a limited exposure for high consumers. However, health risk must not be underestimated as the same samples could contain other such significant mycotoxins as aflatoxins and fumonisins that may interact toxicologically with one another and elicit some synergistic and additive effects on consumers (Cheli et al. 2017). To prevent these mycotoxins exposure risk, there is the need to pay attention on some indicators of the presence of mycotoxins when purchasing spices. Among the quality criteria cited by consumers, the presence of moulds is definitely the most pertinent indicator of the possible presence of mycotoxins. The change of colour and odour can be considered as secondary since they are often the results of this fungal growth. Another critical consumers’ practice identified which may increase the risk of mycotoxin contamination of spices is during storage. In fact, storage at room temperature in tropical climates and for prolonged periods is suitable for the growth of ochratoxinogenic fungi and subsequent OTA production (Esteban et al. 2004; Pardo et al. 2004; Ribeiro et al. 2006; Passamani et al. 2014). Besides, OTA is a thermally stable compound up to 180 °C (Raters and Matissek 2008), which is slightly soluble in water (El Khoury et al., 2010). Thus, measures that are applied by some consumers, which consist of drying or washing spices before use, are not likely to reduce OTA content in these spices. There is therefore a need to ensure that handling of spices during storage is done under conditions that limit fungal growth and mycotoxin synthesis.

Many mitigation strategies have been described as useful to reduce mycotoxins in food and feeds (Karlovsky et al. 2016; Cheli et al. 2017; Chilaka et al. 2017). In the case of our studied spices, simple actions like sorting mouldy grains, proper drying, use of appropriate packaging materials and of proper storage methods and facilities may be helpful in limiting contamination. Unfortunately, up to 88% of our survey respondents indicated they are not aware of the potential presence of mycotoxin in spices. A good starting point should therefore be the enhancement of their knowledge and awareness about what mycotoxins are, the human health risk they pose and preventive measures. Government bodies, private organizations, non-governmental organizations, media (in electronic or printed forms) can therefore play a key role in attaining this objective (Zain 2011).

Conclusion

Taking into consideration the maximum OTA content regulated in spices by the European Commission, and the concentrations recovered from the analyzed samples coupled with the amount consumed daily, there appears to be a limited health risk directly associated with OTA contamination in black pepper, white pepper and clove sold in Yaoundé markets. However, efforts should be made to enhance consumers’ awareness on mycotoxins and associated impact on health, and to enable consumers adopt mycotoxins preventive measures.