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Table 2 All parameters used for the comparison between four methods (M1, M2, M3 and M4) and the final selection of the test suitable method

From: Assessment of antibiotic residues in commercial and farm milk collected in the region of Guelma (Algeria)

Group and type of antibiotic

Spiked Concentration (μg/L)

Average Rec% ± STDEV M1

Average Rec% ± STDEV M2

Average Rec% ± STDEV M3

Average Rec% ± STDEV M4

MRL in milk (μg/L)

MRL in meat (μg/L)

MRL milk/MRL meat

Macrolides

 

35.65 ± 2.72

24.50 ± 8.54

73.66 ± 8.33

79.20 ± 6.51

   

Erythromycin

2000

3.40ab ± 0.98

0.67a ± 0.35

20.10b ± 3.77

0.00a ± 0.00

40

200

0.2

Neospyramycin

2500

18.78 ± 1.33

22.14 ± 1.10

70.31 ± 11.95

102.99 ± 3.17

200

200

1

Spiramycin

2500

25.96 ± 3.06

29.07 ± 1.18

53.17 ± 4.80

37.45 ± 2.02

200

200

1

Tilmicosin

500

96.42 ± 2.34

63.54 ± 39.90

171.49 ± 11.93

245.02 ± 27.02

50

50

1

Tylosin

1000

33.69a ± 5.89

7.08b ± 0.16

53.23a ± 9.22

10.55b ± 0.35

50

100

0.50

Penicillins

 

34.36 ± 2.10

30.36 ± 1.87

72.33 ± 9.17

75.42 ± 7.91

   

Amoxicillin

500

57.08a ± 4.54

69.32a ± 0.38

143.26b ± 25.38

107.28c ± 35.83

4

50

0.08

Ampicillin

500

78.19a ± 7.09

104.03a ± 7.99

204.73b ± 26.17

273.58c ± 7.65

4

50

0.08

Cloxacillin

30,000

1.43a ± 0.12

1.31a ± 0.03

3.05a ± 0.21

5.76a ± 0.49

30

300

0.1

Dicloxacillin

3000

9.13a ± 0.44

6.96a ± 0.09

21.14ab ± 2.80

60.03b ± 5.54

30

300

0.1

Nafcillin

3000

8.23a ± 0.20

10.69a ± 0.12

18.07a ± 0.39

10.23a ± 0.31

30

300

0.1

Oxacillin

3000

10.99a ± 0.86

11.72a ± 0.27

28.44ab ± 1.39

53.30b ± 2.03

30

300

0.1

Penicillin G

500

75.48a ± 1.44

8.46b ± 4.25

87.64a ± 7.85

17.76b ± 3.53

4

50

0.08

Quinolones

 

37.05 ± 2.20

26.58 ± 1.62

76.52 ± 1.74

83.07 ± 13.94

   

Enrofloxacin

500

53.28 ± 3.60

40.40 ± 1.90

110.21 ± 1.22

95.99 ± 19.05

100

100

1

Flumequine

1000

9.05a ± 1.45

0.00b ± 0.00

24.33a ± 0.70

52.72c ± 9.48

50

200

0.25

Marbofloxacin

750

48.80a ± 1.55

39.32a ± 2.94

95.02b ± 3.29

100.49b ± 13.30

75

150

0.5

Sulfonamides

 

34.80 ± 1.32

29.49 ± 2.52

94.58 ± 6.43

99.73 ± 9.82

   

Sulfacetamide

1000

18.54 ± 1.36

14.02 ± 1.57

35.42 ± 8.03

20.53 ± 2.80

100

100

1

Sulfachloropyridazine

1000

38.38 ± 0.48

26.78 ± 3.87

108.43 ± 0.11

132.90 ± 3.87

100

100

1

Sulfadiazine

1000

25.49 ± 2.58

24.09 ± 0.89

58.88 ± 0.63

65.94 ± 3.54

100

100

1

Sulfadimethoxine

1000

14.31 ± 1.46

7.10 ± 0.40

41.56 ± 1.33

85.52 ± 2.47

100

100

1

Sulfadimidine

1000

39.33 ± 0.21

38.29 ± 1.55

114.46 ± 7.31

123.00 ± 9.00

100

100

1

Sulfadoxine

1000

30.31 ± 0.74

16.97 ± 2.60

82.33 ± 0.96

153.84 ± 1.11

100

100

1

Sulfaguanidine

1000

18.64 ± 0.12

14.25 ± 0.14

39.51 ± 7.71

26.60 ± 10.07

100

100

1

Sulfamerazine

1000

9.70 ± 1.57

7.65 ± 0.11

39.23 ± 1.08

89.31 ± 11.19

100

100

1

Sulfamethizole

1000

42.44 ± 0.80

55.31 ± 7.74

92.82 ± 1.55

84.97 ± 4.78

100

100

1

Sulfamethoxazole

1000

43.95 ± 6.02

18.70 ± 3.82

127.40 ± 4.92

161.97 ± 15.02

100

100

1

Sulfamethoxypyridazine

1000

43.94 ± 0.02

51.72 ± 1.76

140.44 ± 5.21

165.70 ± 12.54

100

100

1

Sulfamonomethoxine

1000

42.22 ± 1.56

50.56 ± 0.46

148.67 ± 9.67

174.34 ± 7.26

100

100

1

Sulfamoxole

1000

38.61 ± 1.46

19.51 ± 2.34

80.30 ± 10.71

100.31 ± 23.38

100

100

1

Sulfapyridine

1000

29.02 ± 0.68

31.62 ± 2.64

89.32 ± 6.45

101.35 ± 18.40

100

100

1

Sulfaquinoxaline

1000

13.64 ± 0.32

10.28 ± 2.07

28.72 ± 5.89

79.34 ± 14.84

100

100

1

Sulfathiazole

1000

36.39 ± 1.95

39.05 ± 0.88

88.64 ± 5.73

41.14 ± 21.28

100

100

1

Sulfisoxazole

1000

55.57 ± 0.80

29.05 ± 2.59

168.29 ± 12.57

62.20 ± 4.43

100

100

1

Trimethoprim

1000

86.00 ± 1.57

75.98 ± 9.94

217.95 ± 25.88

126.27 ± 10.81

50

50

1

Tetracyclines

 

25.73 ± 0.54

21.89 ± 17.13

73.50 ± 12.79

52.61 ± 7.69

   

Chlortetracycline

1000

18.87 ± 0.49

21.77 ± 9.36

30.30 ± 6.29

35.67 ± 6.17

100

100

1

Oxytetracycline+ 4-Epi-Oxytetracycline

1000

32.79 ± 0.77

22.24 ± 23.07

108.49 ± 15.90

48.16 ± 2.89

100

100

1

Tetracycline+ 4-Epi-Tetracycline

1000

25.53 ± 0.36

21.67 ± 18.97

81.72 ± 16.19

74.02 ± 14.00

100

100

1

  1. Means with the same letter do not differ significantly at the level of 0.05
  2. STDEV Standard Deviation, Rec Recovery
  3. *the estimated concentration was based on the calibration curve produced with the five different standard concentrations (250, 500, 1000, 1500 and 2000 μg/L) after screening the quantifier ion in the MS spectra. Four methods used (n = 2) in milk samples spiked each antibiotic of 500 μg/L as a final concentration