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Table 2 All parameters used for the comparison between four methods (M1, M2, M3 and M4) and the final selection of the test suitable method

From: Assessment of antibiotic residues in commercial and farm milk collected in the region of Guelma (Algeria)

Group and type of antibiotic Spiked Concentration (μg/L) Average Rec% ± STDEV M1 Average Rec% ± STDEV M2 Average Rec% ± STDEV M3 Average Rec% ± STDEV M4 MRL in milk (μg/L) MRL in meat (μg/L) MRL milk/MRL meat
Macrolides   35.65 ± 2.72 24.50 ± 8.54 73.66 ± 8.33 79.20 ± 6.51    
Erythromycin 2000 3.40ab ± 0.98 0.67a ± 0.35 20.10b ± 3.77 0.00a ± 0.00 40 200 0.2
Neospyramycin 2500 18.78 ± 1.33 22.14 ± 1.10 70.31 ± 11.95 102.99 ± 3.17 200 200 1
Spiramycin 2500 25.96 ± 3.06 29.07 ± 1.18 53.17 ± 4.80 37.45 ± 2.02 200 200 1
Tilmicosin 500 96.42 ± 2.34 63.54 ± 39.90 171.49 ± 11.93 245.02 ± 27.02 50 50 1
Tylosin 1000 33.69a ± 5.89 7.08b ± 0.16 53.23a ± 9.22 10.55b ± 0.35 50 100 0.50
Penicillins   34.36 ± 2.10 30.36 ± 1.87 72.33 ± 9.17 75.42 ± 7.91    
Amoxicillin 500 57.08a ± 4.54 69.32a ± 0.38 143.26b ± 25.38 107.28c ± 35.83 4 50 0.08
Ampicillin 500 78.19a ± 7.09 104.03a ± 7.99 204.73b ± 26.17 273.58c ± 7.65 4 50 0.08
Cloxacillin 30,000 1.43a ± 0.12 1.31a ± 0.03 3.05a ± 0.21 5.76a ± 0.49 30 300 0.1
Dicloxacillin 3000 9.13a ± 0.44 6.96a ± 0.09 21.14ab ± 2.80 60.03b ± 5.54 30 300 0.1
Nafcillin 3000 8.23a ± 0.20 10.69a ± 0.12 18.07a ± 0.39 10.23a ± 0.31 30 300 0.1
Oxacillin 3000 10.99a ± 0.86 11.72a ± 0.27 28.44ab ± 1.39 53.30b ± 2.03 30 300 0.1
Penicillin G 500 75.48a ± 1.44 8.46b ± 4.25 87.64a ± 7.85 17.76b ± 3.53 4 50 0.08
Quinolones   37.05 ± 2.20 26.58 ± 1.62 76.52 ± 1.74 83.07 ± 13.94    
Enrofloxacin 500 53.28 ± 3.60 40.40 ± 1.90 110.21 ± 1.22 95.99 ± 19.05 100 100 1
Flumequine 1000 9.05a ± 1.45 0.00b ± 0.00 24.33a ± 0.70 52.72c ± 9.48 50 200 0.25
Marbofloxacin 750 48.80a ± 1.55 39.32a ± 2.94 95.02b ± 3.29 100.49b ± 13.30 75 150 0.5
Sulfonamides   34.80 ± 1.32 29.49 ± 2.52 94.58 ± 6.43 99.73 ± 9.82    
Sulfacetamide 1000 18.54 ± 1.36 14.02 ± 1.57 35.42 ± 8.03 20.53 ± 2.80 100 100 1
Sulfachloropyridazine 1000 38.38 ± 0.48 26.78 ± 3.87 108.43 ± 0.11 132.90 ± 3.87 100 100 1
Sulfadiazine 1000 25.49 ± 2.58 24.09 ± 0.89 58.88 ± 0.63 65.94 ± 3.54 100 100 1
Sulfadimethoxine 1000 14.31 ± 1.46 7.10 ± 0.40 41.56 ± 1.33 85.52 ± 2.47 100 100 1
Sulfadimidine 1000 39.33 ± 0.21 38.29 ± 1.55 114.46 ± 7.31 123.00 ± 9.00 100 100 1
Sulfadoxine 1000 30.31 ± 0.74 16.97 ± 2.60 82.33 ± 0.96 153.84 ± 1.11 100 100 1
Sulfaguanidine 1000 18.64 ± 0.12 14.25 ± 0.14 39.51 ± 7.71 26.60 ± 10.07 100 100 1
Sulfamerazine 1000 9.70 ± 1.57 7.65 ± 0.11 39.23 ± 1.08 89.31 ± 11.19 100 100 1
Sulfamethizole 1000 42.44 ± 0.80 55.31 ± 7.74 92.82 ± 1.55 84.97 ± 4.78 100 100 1
Sulfamethoxazole 1000 43.95 ± 6.02 18.70 ± 3.82 127.40 ± 4.92 161.97 ± 15.02 100 100 1
Sulfamethoxypyridazine 1000 43.94 ± 0.02 51.72 ± 1.76 140.44 ± 5.21 165.70 ± 12.54 100 100 1
Sulfamonomethoxine 1000 42.22 ± 1.56 50.56 ± 0.46 148.67 ± 9.67 174.34 ± 7.26 100 100 1
Sulfamoxole 1000 38.61 ± 1.46 19.51 ± 2.34 80.30 ± 10.71 100.31 ± 23.38 100 100 1
Sulfapyridine 1000 29.02 ± 0.68 31.62 ± 2.64 89.32 ± 6.45 101.35 ± 18.40 100 100 1
Sulfaquinoxaline 1000 13.64 ± 0.32 10.28 ± 2.07 28.72 ± 5.89 79.34 ± 14.84 100 100 1
Sulfathiazole 1000 36.39 ± 1.95 39.05 ± 0.88 88.64 ± 5.73 41.14 ± 21.28 100 100 1
Sulfisoxazole 1000 55.57 ± 0.80 29.05 ± 2.59 168.29 ± 12.57 62.20 ± 4.43 100 100 1
Trimethoprim 1000 86.00 ± 1.57 75.98 ± 9.94 217.95 ± 25.88 126.27 ± 10.81 50 50 1
Tetracyclines   25.73 ± 0.54 21.89 ± 17.13 73.50 ± 12.79 52.61 ± 7.69    
Chlortetracycline 1000 18.87 ± 0.49 21.77 ± 9.36 30.30 ± 6.29 35.67 ± 6.17 100 100 1
Oxytetracycline+ 4-Epi-Oxytetracycline 1000 32.79 ± 0.77 22.24 ± 23.07 108.49 ± 15.90 48.16 ± 2.89 100 100 1
Tetracycline+ 4-Epi-Tetracycline 1000 25.53 ± 0.36 21.67 ± 18.97 81.72 ± 16.19 74.02 ± 14.00 100 100 1
  1. Means with the same letter do not differ significantly at the level of 0.05
  2. STDEV Standard Deviation, Rec Recovery
  3. *the estimated concentration was based on the calibration curve produced with the five different standard concentrations (250, 500, 1000, 1500 and 2000 μg/L) after screening the quantifier ion in the MS spectra. Four methods used (n = 2) in milk samples spiked each antibiotic of 500 μg/L as a final concentration