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Table 1 Description of Critical Sampling Location (CSLs), samples, periodicity, microbiological parameters, methodologies, results interpretation and references

From: Insights in agricultural practices and management systems linked to microbiological contamination of lettuce in conventional production systems in Southern Brazil

CSL Description Samples Time Microbiological parameters Methodology Interpretation of the results* References
1 Manure 3 samples T0 E. coli ISO 21528-2:2004 10³ cfu/g MAPA/ IN n°46. (2011)
     E. coli O157:H7 ISO 16654:2001 A/25g ND
     Salmonella spp. ISO 6579:2002 A/25g MAPA/ IN n°46. (2011)
2 Manured soil 3 samples → 3 x 3 pooled T0 E. coli ISO 21528-2:2004 10³ cfu/g MAPA/ IN n°46. (2011)
     E. coli O157:H7 ISO 16654:2001 A/25g ND
     Salmonella spp. ISO 6579:2002 A/25g MAPA/ IN n°46. (2011)
3 Soil 3 samples → 3 x 3 pooled T1 E. coli ISO 21528-2:2004 10³ cfu/g MAPA/ IN n°46. (2011)
    T2 E. coli O157:H7 ISO 16654:2001 A/25g ND
    T3 Salmonella spp. ISO 6579:2002 A/25g MAPA/ IN n°46. (2011)
4 Seedlings in soil 1 sample → 1 x 3 pooled T0 E. coli ISO 21528-2:2004 10² cfu/g RDC n°12 (2001)
     E. coli O157:H7 ISO 16654:2001 A/25g ND
     Salmonella spp. ISO 6579:2002 A/25g RDC n°12 (2001)
5 Seedling 1 sample T0 E. coli ISO 21528-2:2004 10² cfu/g RDC n°12 (2001)
6 Lettuce 3 samples → 3 x 3 pooled T1 E. coli ISO 21528-2:2004 10² cfu/g RDC n°12 (2001)
    T2 E. coli O157:H7 ISO 16654:2001 A/25g ND
    T3 Salmonella spp. ISO 6579:2002 A/25g RDC n°12 (2001)
7 Lettuce after washing 3 samples → 3 x 3 pooled T3 E. coli ISO 21528-2:2004 10² cfu/g RDC n°12 (2001)
     E. coli O157:H7 ISO 16654:2001 A/25g ND
     Salmonella spp. ISO 6579:2002 A/25g RDC n°12 (2001)
8 Rinse water 100 ml T3 E. coli 20 TH APHA (1998) 2 x102 MPN/100ml CONAMA. n°357 de 2005
     E. coli O157:H7 ISO 16654:2001 A/25ml ND
     Salmonella spp. ISO 6579:2002 A/25ml ND
9 Irrigation water source 100 ml T0 T1 E. coli 20 TH APHA (1998) 2 x102 MPN/100ml CONAMA. n°357 de 2005
    T2 E. coli O157:H7 ISO 16654:2001 A/25ml ND
    T3 Salmonella spp. ISO 6579:2002 A/25ml ND
10 Irrigation water from tap 100 ml T0 T1 E. coli 20 TH APHA (1998) 2 x102 MPN/100ml CONAMA. n°357 de 2005
    T2 E. coli O157:H7 ISO 16654:2001 A/25ml ND
    T3 Salmonella spp. ISO 6579:2002 A/25ml ND
11 Swab of farmers’ hands 3 x 25 cm² T3 E. coli ISO 21528-2:2004 and AOAC (1998) ≤ 0.7 log cfu/25 cm² (below detection) Jacxsens. et al. (2010)
12 Swab of transport boxes of lettuce 3 x 50 cm² T3 E. coli ISO 21528-2:2004 ≤ 0.7 log cfu/25 cm² (below detection) Jacxsens. et al. (2010)
  1. A: absent; ND: not defined by official regulation.
  2. T0: At planting. T1: Two weeks before harvest. T2: One week before harvest. T3: At harvest.
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International Journal of Food Contamination

ISSN: 2196-2804

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