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Table 3 Comparison of detection of A. acidoterrestris in different fruit matrices by real-time PCR and conventional plating using K agar and confirmation of guaiacol formation

From: Fruit juice and puree characteristics influence enrichment requirements for real-time PCR detection of Alicyclobacillus acidoterrestris

Sample 0 Brix Time requirement* Growth on K Agar Guaiacol Formation
24 h 48 h 24 h 48 h
Orange concentrate 43 24 h - - - -
Apple concentrate 40 24 h + + + +
Ketchup 27 24 h - - - -
Tomato paste 26 24 h + + + +
Apple puree 17 24 h - + - +
Peach puree 17 24 h - + - +
Apple juice (filtered) 12 24 h - + - +
Peach nectar 12 24 h + + + +
Apple juice (unfiltered) 11 24 h + + + +
Orange juice (with pulp) 11 24 h - + - +
Orange juice (no pulp) 10 24 h - + - +
Tomato sauce 9 24 h + + + +
Tomato juice 6 24 h + + + +
  1. *enrichment time required for positive detection by real-time PCR.
  2. -: not detectable.